Abstract

Halophilic bacteria produce ectoine quite effectively, a vital osmoprotectant and compatible solute, helping them survive extremely high salinity and osmotic shock. Cyclic amino acid garners considerable industrial attention owing mainly to protective properties in various biotechnological, medical, and some cosmetic applications. Microorganisms thriving in salt pans and hypersaline lakes alongside marine ecosystems produce ectoine naturally under extremely salty conditions. Deeper insight into genetic and biochemical mechanisms underlying ectoine biosynthesis, namely ectA, ectB, and ectC gene cluster, has enabled a significant boost in production via metabolic engineering improvements. Although fermentation-based production is still the major technique, other strategies, including synthetic biology, bacterial milking, and adaptive evolution, are becoming viable substitutes. Ectoine is extracted and purified industrially utilizing advanced techniques such as solvent extraction, chromatographic procedures, and membrane-based filtering; research is continuously being conducted to find more economical and environmentally friendly approaches. This review provides a summary of the microbial diversity of ectoine producers, biosynthetic processes, and the most recent technical advancements in its synthesis and industrial applications.