Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae from clinical samples

Shova Shrestha; Prakash Ghimire; Hari Prasad Kattel; Megha Raj Banjara

doi:10.54796/njb.v13i2.465

Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae from clinical samples

Shova Shrestha
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
Prakash Ghimire
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu
Hari Prasad Kattel
Tribhuvan University Teaching Hospital, Maharajgunj, Kathmandu
Megha Raj Banjara
Central Department of Microbiology, Tribhuvan University, Kirtipur, Kathmandu

Abstract

This study aimed to determine the MDR and ESBL producing K. pneumoniae from clinical samples. Laboratory based cross-sectional study was conducted from the samples obtained from the patients of Tribhuvan University Teaching Hospital. Antibiotic susceptibility test was performed and ESBL was detected through the combination disk method. Of the 377 K. pneumoniae isolates, 304 (80.6%) were MDR and 279 (74%) exhibited ESBL activity. Significant percentages of MDR were detected in blood (p=0.004) and urine (p=0.058) whereas ESBL in urine (p=0.026). Most ESBL-producing K. pneumoniae exhibited resistance to ß-lactams, quinolones, aminoglycosides and macrolids. This suggests for strict adherence to antimicrobial susceptibility results for the treatment and implementation of antibiotic stewardship programme.

PDF

Published at

31. December 2025
https://doi.org/10.54796/njb.v13i2.465
Issue:

Vol. 13 No. 2 (2025)
Section:

Original Research Articles
Keywords:

Klebsiella pneumoniae clinical samples MDR ESBL antibiotic susceptibility
Share:

Z

Shrestha, S., Ghimire, P., Kattel, H. P., & Banjara, M. R. (2025). Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae from clinical samples. Nepal Journal of Biotechnology, 13(2), 162–167. https://doi.org/10.54796/njb.v13i2.465

Download Citation

This work is licensed under a Creative Commons Attribution (CC BY) 4.0 International License.

Copyright Notice:

Copyright Notice:
The manuscript submitted to NJB must be an original contribution, not previously published and should not be under consideration for publication elsewhere. When the manuscript is accepted for publication, the authors agree to automatically transfer the copyright of the article to the publisher. It should grant permission to any third party, in advance and in perpetuity, the right to use, reproduce or disseminate your article, according to the NJB copyright and license agreement.

Authors transfer copyright to the publisher as part of a journal publishing agreement but have the rights to: Share their article for Personal Use, Internal Institutional Use and Scholarly Sharing purposes, with the NJB applies the Creative Commons Attribution CC BY 4.0 license to all the works we publish after July 2022. Under this license, authors agree to make articles legally available for reuse, without permission or fees, for virtually any purpose. Anyone may remix, transform, and build upon the material for any purpose, even commercially, and although their new works must also acknowledge you, they don’t have to license their derivative works on the same terms. More details on CC BY 4.0 refer to its Licence Deed and Legal Code.

PlumX

Dimensions

Views:

Total	Abstract	PDF
51	0	51